He was responsible for some of the most popular superhero characters in history including Spider-Man, X Men, the Incredible Hulk and Iron Man.
Lee’s daughter, J.C. Lee, confirmed his death to Reuters news agency, saying that her father “loved his life and loved what he did for a living”.
She added: “His family loved him and his fans loved him. He was irreplaceable.”
Tributes to Lee have poured in from the world of film including messages from actors who starred in Marvel movies.
“There will never be another Stan Lee,” tweeted the actor Chris Evans, who played Captain America on the big screen.
“For decades he provided both young and old with adventure, escape, comfort, confidence, inspiration, strength, friendship and joy.”
Wolverine actor Hugh Jackman said the world had “lost a creative genius”.
Iron Man star Robert Downey Jr posted a photo on Instagram of himself with Lee and an image of a love heart.
“I owe it all to you, Rest in Peace Stan.” He wrote
Lee, a writer and editor, worked with artists such as Jack Kirby and Steve Ditko to turn Marvel into a major comic book brand in the 1960s.