The Trump administration is set to reinstate all US sanctions on Iran removed under the 2015 nuclear deal.
The White House said it was “the toughest sanctions regime ever imposed on Iran” and targeted Iran’s energy, shipping and banking sectors.
However, eight countries will not be penalized by the US for continuing to import Iranian oil.
In a joint statement, the foreign ministers of the UK, Germany and France, and EU foreign affairs chief Federica Mogherini said they “deeply” regretted the US decision to restore sanctions.
“We are determined to protect European economic operators engaged in legitimate business with Iran, in accordance with EU law and with UN Security Council resolution 2231,” they said.
An Iranian foreign ministry spokesman said Iran was unconcerned at the return of sanctions.